3D Reconstruction

The Gatan Reconstruction Software provides tools to precisely align the 2D projections collected by the Tomography Software and generate a 3D volume from the aligned projections. It is strongly recommended that this software be run on a system with at least 2GB of RAM. Specific features are:

• Manual and Automatic tilt series alignment with sub-pixel accuracy
• Assisted determination of tilt axis location
• Multiple reconstruction algorithms including Weighted Back Projection (WBP) and the Simultaneous Iterative Reconstruction Technique (SIRT)
• Memory conserving version of the WBP and SIRT Algorithms
• Support for sub-volume reconstruction
• Live updates of volume during reconstruction

Comparison of reconstruction algorithms from simulated data. The left image shows the original object, a cube. Projections were generated from -60 degrees to +60 degrees at 1 degree intervals. The middle image shows the cube reconstructed using weighted back projection. The right image shows the reconstruction using 20 iterations of the SIRT algorithm. The blue and red levels correspond to 30% and 60% of the maximum level in the reconstruction.

Product Requirements